Primer Premier 6.1

Primer Premier is a comprehensive primer design application.PCR primer design- design primer pairs for PCR and multiplex experiments. Cross species- Amplify sequences from multiple species.Pathogenic detection- Locate oligos in highly conserved regions. Allele specific- Exclusively amplify a single member of a set of related sequences.Degenerate- Start with an amino acid sequence, reverse translate and design oligos in regions of low degeneracy automatically.Nested/multiplex- Checking homology among multiple candidate oligos. Restriction enzyme and motif analysis- Select from the database of over 800 enzymes and 200 common motifs. Clustal W alignments- Load the aligned sequences after performing ClustalW alignment using any resource, including EBI.Multiple sequence alignment- Use the popular Clustal W algorithm to align sequences from your desktop (available in Windows version only).Algorithm- Calculates Tm using Breslauer values. Long PCR- Handles sequences up to 50 KB.